5 LABORATORY DIAGNOSIS | 5B BLOOD COUNT AND MORPHOLOGY | a) Automated complete blood count with white blood cell differential |
5 LABORATORY DIAGNOSIS | 5B BLOOD COUNT AND MORPHOLOGY | b) Performing aspiration and biopsy of bone marrow, lumbar puncture and lymph node fine needle aspiration |
5 LABORATORY DIAGNOSIS | 5B BLOOD COUNT AND MORPHOLOGY | c) Preparation, fixation, staining, reading and reporting of peripheral blood smears and bone marrow aspirates, and trephine rolls |
5 LABORATORY DIAGNOSIS | 5B BLOOD COUNT AND MORPHOLOGY | d) Cytochemical, special stains and immunostaining of blood and bone marrow smears in hematological conditions |
5 LABORATORY DIAGNOSIS | 5C OTHER LABORATORY TECHNIQUES | a) Hemoglobin analyses (e.g., hemoglobin electrophoresis) |
5 LABORATORY DIAGNOSIS | 5C OTHER LABORATORY TECHNIQUES | b) Other red blood cell laboratory techniques (e.g., sickling process, oxygen affinity, red blood cell enzyme assays – pyruvate kinase, glucose-6-phosphate dehydrogenase) |
5 LABORATORY DIAGNOSIS | 5C OTHER LABORATORY TECHNIQUES | c) Laboratory work-up on iron metabolism and vitamin deficiencies |
5 LABORATORY DIAGNOSIS | 5C OTHER LABORATORY TECHNIQUES | d) Detection of immunoglobulin abnormalities |
5 LABORATORY DIAGNOSIS | 5C OTHER LABORATORY TECHNIQUES | e) Progenitor quantification in semi-solid culture conditions |
5 LABORATORY DIAGNOSIS | 5D IMMUNOPHENOTYPING BY FLOW CYTOMETRY | a) Clinical applications of flow cytometry for diagnosis, classification, prognosis, evaluation of minimal residual disease and stem cell quantification |
5 LABORATORY DIAGNOSIS | 5D IMMUNOPHENOTYPING BY FLOW CYTOMETRY | b) Pre-analytical and analytical phase of flow cytometry of blood, bone marrow, and body fluids (e.g., specimen processing, surface vs. intracytoplasmic staining, acquiring data, gating strategies) |
5 LABORATORY DIAGNOSIS | 5D IMMUNOPHENOTYPING BY FLOW CYTOMETRY | c) Essential cellular markers, disease-oriented antibody panels applied in hematological conditions |
5 LABORATORY DIAGNOSIS | 5D IMMUNOPHENOTYPING BY FLOW CYTOMETRY | d) Data analysis and interpretation (e.g. determination of the lineage of cells of interest, clonality, stem cell quantification, telomere length and specific subtypes of hematological condition) |
5 LABORATORY DIAGNOSIS | 5E GENETICS AND MOLECULAR BIOLOGY | a) Clinical applications of these techniques for diagnosis, classification, prognosis, minimal residual disease of hematological disorders |
5 LABORATORY DIAGNOSIS | 5E GENETICS AND MOLECULAR BIOLOGY | b) Conventional cytogenetics, chromosome breakage and fluorescence in situ hybridization |
5 LABORATORY DIAGNOSIS | 5E GENETICS AND MOLECULAR BIOLOGY | c) Polymerase chain reactions for the detection of gene mutations, fusion genes, clonality assessment, and gene expression |
5 LABORATORY DIAGNOSIS | 5E GENETICS AND MOLECULAR BIOLOGY | d) Other techniques for detection of copy number variations and gene polymorphisms |
5 LABORATORY DIAGNOSIS | 5E GENETICS AND MOLECULAR BIOLOGY | e) Other techniques for detection and quantification of recurrent mutations |
5 LABORATORY DIAGNOSIS | 5F COAGULATION | a) Techniques for assessing coagulation and platelets |
5 LABORATORY DIAGNOSIS | 5F COAGULATION | b) Assays for inhibitors |
5 LABORATORY DIAGNOSIS | 5F COAGULATION | c) Assays for monitoring anticoagulants |
5 LABORATORY DIAGNOSIS | 5B BLOOD COUNT AND MORPHOLOGY | e) Review and interpretation of trephine, lymph node and other relevant tissue biopsies together with a pathologist |
5 LABORATORY DIAGNOSIS | 5B BLOOD COUNT AND MORPHOLOGY | f) Pseudo thrombocytopenia |
5 LABORATORY DIAGNOSIS | 5E GENETICS AND MOLECULAR BIOLOGY | f) Other techniques for gene discovery and expression |
5 LABORATORY DIAGNOSIS | 5G IMMUNOHEMATOLOGY | a) Red cell typing and allocation |
5 LABORATORY DIAGNOSIS | 5G IMMUNOHEMATOLOGY | b) Minor RBC, platelet and neutrophil antigens |
5 LABORATORY DIAGNOSIS | 5G IMMUNOHEMATOLOGY | c) Laboratory diagnosis of alloimmune and autoimmune cytopenia |